Study of genetic structure of honey bee from cyprus apis. In this study, insecticide incidence, dna damage, and antioxidant enzyme activity were studied intrareas. Newly emerged worker bees were exposed to organophosphate insecticide coumaphos, a neonicotinoid imidacloprid, and their mixtures. In the present study, honeybees were irradiated right after birth for 14 days with gamma rays at dose rates ranging between 4. Several physical, biochemical and antioxidant properties of two nigerian stingless bee honey varieties melipona sp. Various detoxifying enzymes, including microsomal oxidases, glutathione stransferases, esterases, epoxide hydrolase, and ddtdehydrochlorinase, were assayed in adult worker bees apis mellifera l.
Antioxidant enzymes in the honey bee, apis mellifera gunter weirich, anita collins, virginia williams to cite this version. May 28, 20 as a managed pollinator, the honey bee apis mellifera is critical to the american agricultural enterprise. Physicochemical and antioxidant properties of malaysian. The most important antioxidant enzymes identified in honeybee body fluids are superoxide dismutase sod, catalase cat, glutathione. What is the main driver of ageing in longlived winter. Request pdf sperm storage and antioxidative enzyme expression in the honey bee, apis mellifera honey bee apis mellifera sperm remains viable in the spermatheca of mated female honey bees for. Modification of the head proteome of nurse honeybees apis.
Tolerance and response of two honeybee species apis. Antioxidant, antihyperglycemic, and antidiabetic activity. Although this parasite is presently spreading across the world into its novel host, the mechanisms by it which affects the bees and how bees respond are not well understood. The difference in floral sources, in these two localities, might explain the difference in antibacterial effects of apis mellifera tigray white and gondar yellow honeys. Sister worker honeybees apis mellifera produced by a single queen bee were sampled at the beginning november 2010, approximately 6 weeks old and in the middle of winter january 2011, approximately 12 weeks old from one colony at the university apiary clujnapoca. Formetanate toxicity and changes in antioxidant enzyme system. Social living bees have been domesticated to generate bee products in sufficient. Terrestrial ecosystems are exposed to various kinds of pollutants, including radionuclides. Honeybee antioxidant enzymes are of particular interest because of their. The honeybee, apis mellifera, is commonly used in ecotoxicology as a model species for evaluating the effects of pollutants. Antibacterial effects of apis mellifera and stingless bees. The protective strategy of antioxidant enzymes against hydrogen peroxide in honey bee, apis mellifera during two different seasons article pdf available january 2012 with 228 reads how we.
Transcriptomic analysis reveals apis mellifera adaptations. Recent declines in bee populations have prompted numerous studies on the. Honey bees collect floral nectar and pollens, for feeding, which. The most important antioxidative enzymes in the insect are superoxide dismutase sod. Curcumin stimulates biochemical mechanisms of apis. This study was to observe the developmental regulation of cuznsod, mnsod and cat genes in apis mellifera. Pineapple honey is also produced by the apis mellifera bees that collect the nectar of pineapple ananas comosus flowers, resulting in a honey with a distinctive aroma and flavor. Nov 21, 2017 the western honey bee apis mellifera is a eusocial species whose foragers collect food to meet hive requirements and adjust their foodgathering behavior according to these collective needs. The objective of this study was to research the effect of six dietary ala levels on the growth and development of apis mellifera ligustica colonies. Evans usdaars bee research laboratory, beltsville, md, usa abstract honey bee apis mellifera sperm remains viable in the spermatheca of mated female honey bees for several years. Behavioral responses of honey bees apis mellifera to. Changes in mrna expression level of antioxidant enzyme. Unexpectedly strong effect of caffeine on the vitality of. Among other factors, the success of bee colonies depends on health of developed larvae.
Pdf the protective strategy of antioxidant enzymes against. To develop a fundamental and comprehensive understanding of lipids in rj, this. The superoxide dismutase and catalase play an important role in protecting worker bees from reactive oxygen species, and their enzyme activities and expression levels are associated with honeybee longevity and disease resistance which affect the performance of the colony. Apis mellifera bee tea is used by indigenous brazilians to treat diabetes, and this traditional knowledge needs to be recorded and studied. Bee venom bv can be produced by all species of honey bees, e. Using mitochondria isolated from the thoraces, complete oxidation of most of the tca cycle members has been shown. Toxicity of oxalic acid and impact on some antioxidant. The foraging behaviour of honey bees, apis mellifera. Mic of white and yellow honeys for 40% of the test organisms was 6. The protective strategy of antioxidant enzymes against. Although modern dictionaries may refer to apis as either honey bee or honeybee, entomologist robert snodgrass asserts that correct usage requires two words, i. The honeybee, apis mellifera, is commonly used in ecotoxicology as a model species for evaluating the effects of. Antioxidant enzymes in the honey bee, apis mellifera.
The objective of this study was to record the use and to evaluate the antioxidant, antihyperglycemic, and antidiabetic activity of apis mellifera. Curcumin stimulates biochemical mechanisms of apis mellifera. Glutathione stransferases gsts are an important family of multifunctional enzymes in aerobic organisms. This behaviour is the link between the honey bee colony and the ambient environment. Physiological effects of gamma irradiation in the honeybee. Sperm storage and antioxidative enzyme expression in the honey bee, apis mellifera. In this study, we explored the effects of higher temperature and humidity on the epiphysiology of bees by evaluating the survival, tolerance and body water loss in two bee species apis cerana and apis mellifera. Formetanate toxicity and changes in antioxidant enzyme system of apis mellifera larvae. Tolerance and response of two honeybee species apis cerana. The substantial activities of all three enzymes in spermathecae of mated queens suggest their involvement in the longterm protection of the spermatozoa from oxidative stress. Based on this, corona and robinson 2006 showed at least 38 genes coding for antioxidative proteins. The protective strategy of antioxidant enzymes against hydrogen peroxide in honey bee, apis mellifera during two different seasons. Nontarget inhibition of antioxidant enzymes in honey bees a. Nontarget inhibition of antioxidant enzymes in honey bees.
Pdf the protective strategy of antioxidant enzymes. Aboushaara faculty of agriculture, damanhour university, damanhour, egypt abstract. Substantial percentage of world food production depends on pollinating service of honeybees that directly depends on their health status. We also evaluated the changes in the activity of antioxidant and detoxification enzymes. Acacia and pineapple honey are produced by apis mellifera bees while borneo and tualang honey are produced by apis cerana and apis dorsata bees, respectively. Request pdf toxicity of oxalic acid and impact on some antioxidant enzymes on in vitroreared honeybee larvae nowadays, varroa destructor is considered as a serious pest of honeybees apis. Honey bee apis mellifera drones survive oxidative stress. Supplementation of the honey bee diet with vitamin c.
The basic biology of apis mellifera the western honeybee. Antioxidant properties of honey and its role in preventing. Both these enzymes are part of the primary antioxidant response and act. The crucial phase of larval development is the first 6 days after hatching when a worker larva grows exponentially and larvae are potentially exposed to xenobiotics via diet. Antioxidant enzymes in the honey bee, apis mellifera apidologie. Antioxidant activity and irritation property of venoms. Apis mellifera are important pollinating bees that are widely used in agricultural systems. Honey is the only food that includes all the substances necessary to sustain life, including enzymes. In this study, we assayed reactive oxygen species ros and antioxidant enzymes. Honey bee apis mellifera drones survive oxidative stress due to increased tolerance instead of avoidance or repair of oxidative damage hongmei libyarlaya,b,c.
Antioxidant enzymes in the honey bee, apis mellifera article pdf available in apidologie 331 january 2002 with 918 reads how we measure reads. Sperm storage and antioxidative enzyme expression in the. Three honey bee samples s, of 30 g honey bees each, were collected from each sz for the honey bee species a. We used sister worker offspring of a single queen to minimize genetic. Differential expression of mitochondrial genes between queens and workers during caste determination in the honeybee apis mellifera. The honey bee apis mellifera, the test species used in the current environmental risk assessment procedure, is generally considered as extremely sensitive to pesticides when compared to other bee. Apis mellifera, apis cerena, apis florea, and apis dorsata. Evaluation of physicochemical and antioxidant properties of. Nutritional effect of alphalinolenic acid on honey bee. The species apis mellifera, belonging to the subfamily apinae, known as the european honey bee, is exotic in brazil and is a major producer of propolis, which has been reported to present important antioxidant, antimicrobial 14,29 and antitumour 30,31 activities. Effects of flight activity and age on oxidative damage in.
For the honey bee apis mellifera the assessment of antioxidative capability became easier after the sequencing of the honey bee genome. The aim of this work was to evaluate enzyme kinetics and inhibition by insecticides in apis mellifera and apis florea. Of the two species, the honeybee has by far the more elaborately organised life. Contribution of lipids in honeybee apis mellifera royal. Nowadays, varroa destructor is considered as a serious pest of honeybees apis mellifera and its resistance to acaricides has been reported in europe since the early 1990s. We examined the influence of caffeine on honeybee lifespan, nosema resistance, key enzyme activities, metabolic compound concentrations, and total dna methylation levels. Detoxification mechanisms of honey bees apis mellifera. Supplementing with vitamin c the diet of honeybees apis mellifera carnica parasitized with varroa destructor. Understanding how different insecticides influence bee health is very essential. During this time, the sperm retains respiratory activity, placing it at risk of the damaging effects of reactive oxygen species common to many biological processes. During this time, the sperm retains respiratory activity, placing it at risk of the damaging.
We determined that constituents found in honey, including p coumaric acid, pinocembrin, and pinobanksin 5methyl ether. Catalase cat, glutathione stransferase gst and superoxide dismutase sod activities were determined in postmitochondrial fractions of. Each colony contained a young normal egglaying queen and had a working population of eight frames of comb with larvae, pupae, honey, and pollen dai et al. The western honey bee or european honey bee apis mellifera is the most common of the 712 species of honey bees worldwide. Accepted, 304 accepted, 304 a gricultural r esearch s ervice, b eltsville a rea b ee r esearch l aboratory. Evans usdaars bee research laboratory, beltsville, md, usa abstract honey bee apis mellifera. Archive ouverte hal antioxidant enzymes in the honey bee, apis mellifera. Antioxidative enzymes might help reduce this damage. Feb 23, 20 acacia honey is produced by apis mellifera, a cultured bee that harvests the extrafloral nectar of acacia mangium trees. The honey bee apis mellifera is an ecologically and. Honey constituents upregulate detoxification and immunity. This natural product is widely appreciated as the only concentrated form of sugar available. Effects of flight activity and age on oxidative damage in the honey.
Tested enzymes showed variable affinity to substrates. The honey bee apis mellifera is an ecologically and economically important pollinator species worldwide. The genus name apis is latin for bee, and mellifera is the latin for honey. Moreover this organisation has been surprisingly little altered by the \domestication of the bees. Honey is nectar collected from many plants and processed by honey bees apis mellifera.
Gut pathology and responses to the microsporidium nosema. Total antioxidant capacity of honeybee haemolymph in relation to. In this study, we assayed reactive oxygen species ros and antioxidant enzymes in the trophocytes and fat cells of young and old queens. Honey bees, apis mellifera, are markedly less sensitive to neonicotinoid insecticides containing a cyanoimino pharmacophore than to those with a nitroimino group. Oxidative stress and antioxidant enzyme activities in the trophocytes and fat cells of queen honeybees apis mellifera yushan hsieh 2 and chinyuan hsu 1, 2 1 department of biomedical. Honey bees, scientifically also known as apis mellifera, which mean honeycarrying bee, are environmentally friendly and are vital as pollinators. Oxidative stress and antioxidant enzyme activities in the. Foraging and pollination activity of apis mellifera adansonii. May 14, 2019 korayem am, khodairy mm, abdelaal aaa, elsonbaty aam 2012 the protective strategy of antioxidant enzymes against hydrogen peroxide in honey bee, apis mellifera during two different seasons. It is the only insect that produces food eaten by man. Flumethrin at honeyrelevant levels induces physiological. Honey bee apis mellifera sperm remains viable in the spermatheca of mated female honey bees for several years. Effects of coumaphos and imidacloprid on honey bee. Oct 19, 20 when apis mellifera honey eucalyptus and stingless bees honey were compared, the former had higher phenolic and flavonoid contents than the stingless bees honey, which in turn had the higher antioxidant activity.
Antioxidant properties of honey act as an antidepressant during high emotional, physical and intellectual stress 17. Chemical communication in the honeybee apis mellifera l. The protective strategy of antioxidant enzymes against hydrogen peroxide in honey bee, apis mellifera during two different seasons by ahmad m. Although previous work has suggested that this results from enhanced metabolism of the former by detoxification enzymes, the specific enzyme. Classification of the protein sequences showing decreased expression p apis mellifera exposed to fieldrelevant doses of the fungicide pyraclostrobin and insecticide.
Insects have a range of antioxidant enzymes, mainly composed of superoxide dismutase sod, catalase cat, and peroxidase pod. They play a crucial role in the detoxification of exogenous compounds, especially. Pdf antioxidant enzymes in the honey bee, apis mellifera. However, the higher temperatures and humidity in greenhouses are not conducive to the survival of bees. To understand how the physiological and cellular mechanisms that determine the onset and duration of senescence are shaped by behavioral development and behavioral duration, we exploited the tractability of the honey bee apis mellifera model system. Formetanate toxicity and changes in antioxidant enzyme. Apr, 2017 substantial percentage of world food production depends on pollinating service of honeybees that directly depends on their health status. The honeybee apis mellifera belongs to the hymenoptera insects that live in social groups with a fixed function of individuals including their rotation during the ontogeny of individuals, resulting in a swarm, which works to some extent, as an organism. Antioxidant and cytotoxic activity of propolis of plebeia. Abstract catalase cat, glutathionestransferase gst and superoxide dismutase sod activi ties were determined in postmitochondrial. Honeybee apis mellifera royal jelly rj has a long history in human medicine because of its healthprotecting properties. The aim of this study was to evaluate the antibacterial effects of ethiopian honeys against antibiotic susceptible and. Apoidae on fruit and seed of allium cepa its foraging and pollinating activities were studied in maroua, from november 2010 to april 2011 and 2012.
Foraging behaviour is one of the distinctive behaviours of honey bees, apis mellifera. Alphalinolenic acid ala, which is an n3 polyunsaturated fatty acid pufa, influences honey bee feed intake and longevity. High levels of these indicators ensure a high level of antioxidants in honey. Honey bee rearing, collection and dissection honey bees apis mellifera linnaeus 1758 for this study were reared at the university of nevada, las vegas unlv, nv, usa apiary. Oxidative dissimilation has been studied in enzymes from the honey bee. Frequent and highly aerobic behaviors likely contribute to naturally occurring stress, accelerate senescence and limit lifespan. The microsporidium nosema ceranae is a newly prevalent parasite of the european honey bee apis mellifera. Transcriptomic analysis reveals apis mellifera adaptations to high temperature and high humidity.1601 1540 904 106 1279 1531 1033 705 535 373 1218 1164 241 1070 1274 541 12 528 1472 1244 166 1267 1063 313 594 1495 1036 1481 51 1170 129 1293 297 380 1217 1215 1436